A2668

Immunoblotting
Chicken gizzard cells were separated on SDS-PAGE and probed with AB to Actin (RB) (Cat. No. A2668). The antibody was developed with HRP APA Rabbit IgG conjugate (Cat. No. A0545) and an AEC substrate.
Antibody concentration 1/200.

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A2668

Sigma

 

Anti-Actin antibody produced in rabbit

whole antiserum

MDL number:MFCD00145889

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Description

Immunogenactin from chicken back muscle
Other NotesTo view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
SpecificityBy immunoblotting, cross-reactivity with other proteins may be observed.
ApplicationMay be used for studying the intracellular distribution of actin structures and their functions in the contractile system.

Properties

antibody formwhole antiserum
clone(polyclonal)
contains15 mM sodium azide
species reactivityrat, chicken, human, mouse
application(s)immunoblotting: suitable using rabbit skeletal muscle α-actin1
 indirect immunofluorescence: 1:40 using cultured chicken fibroblasts
Gene Informationhuman ... ACTA1(58)
 rat ... Acta1(29437)
 mouse ... Acta1(11459)
shipped indry ice
storage temp.−20°C

Safety

WGK Germany3

References

referenceKayalar, C., et al. Proc. Natl. Acad. Sci. U. S. A. 93, 2234, (1996) Abstract
 Fan, A.C., et al., Inhibition of HMGcoA reductase by atorvastatin prevents and reverses MYC-induced lymphomagenesis Blood 110, 2674-2684, (2007) Abstract
 Risco, C., et al., Endoplasmic Reticulum-Golgi Intermediate Compartment Membranes and Vimentin Filaments Participate in Vaccinia Virus Assembly J. Virol. 76, 1839-1855, (2002) Abstract
 Vallee, B.L., et al., Actin is a binding protein for angiogenin. Proc. Natl. Acad. Sci. U. S. A. 90, 1217-1221, (1993) Abstract